Both positive and negative regulatory elements mediate expression of a photoregulated CAB gene from Nicotiana plumbaginifolia.
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چکیده
منابع مشابه
Cytokinins and auxins control the expression of a gene in Nicotiana plumbaginifolia cells by feedback regulation.
Both cytokinin (N6-benzyladenine [BA]) and auxin (2,4-dichlorophenoxyacetic acid [2,4-D]) stimulate the accumulation of an mRNA, represented by the cDNA pLS216, in Nicotiana plumbaginifolia suspension culture cells. The kinetics of RNA accumulation were different for the two hormones; however, the response to both was transient, and the magnitude of the response was dose dependent. Runoff trans...
متن کاملTissue-Specific and Pathogen-lnduced Regulation of a Nicotiana plumbaginifolia
The Nicotiana plumbaginifolia gnl gene encoding a 8-1,3-glucanase isoform has been characterired. The gnl product represents an isoform distinct from the previously identified tobacco 8-1,3-glucanases. By expressing gnl in Escherichia coli, we have determined directly that the encoded protein does, indeed, correspond to a &1,3glucanase. In N. plumbaginifolia, gnl was found to be expressed in ro...
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Most enhancer elements so far described are comprised of multiple DNA binding sites for proteins that act to increase the rate of transcription. In this report we show that the far upstream mouse albumin enhancer element (-10.5 to -8.5 kilobase pairs) is a composite of at least three functional regions: a negative region that suppresses an otherwise positive, liver-specific enhancer element and...
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The B-Peru allele of the maize b regulatory gene is unusual relative to most b alleles in that it is expressed in the aleurone layer of the seed. It is also expressed in a subset of plant vegetative tissues. Transgenic maize plants containing the B-Peru gene with the first 710 bases of upstream sequence conferred the same levels of aleurone expression as nontransgenic B-Peru plants, but no pigm...
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The two tissue-specific mRNAs encoding the isozymes of rabbit angiotensin-converting enzyme (ACE) are generated from the same gene by alternative choice of two transcription initiation sites 5.7 kb apart. In the current study, we have characterized the regulatory sites controlling the transcription of the larger pulmonary isozyme mRNA. For this purpose, reporter genes driven by varying lengths ...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1988
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1988.tb03030.x